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lentiviral vector  (Addgene inc)


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    Structured Review

    Addgene inc lentiviral vector
    Lentiviral Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna5+vector/pcDNA5%2FFRT%2FTO+HIS+DNAJB2a+(Plasmid+%2319550)/bio_rxiv__64898__2025__12__10__693579-303-9-12
    Average 93 stars, based on 4 article reviews
    lentiviral vector - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Clone Assay:

    Article Title: PHC1 maintains pluripotency by organizing genome-wide chromatin interactions of the Nanog locus.
    Article Snippet: The coding sequence of Nanog gene was amplified from cDNA obtained from mESCs and cloned into the PiggyBac transposon pPB-CAG-3×FLAG-pgkhph vector, a gift from Jin Zhang (Zhejiang University School of Medicine, China). .. HA-NANOG and FLAG-PHC1 sequences were cloned into pcDNA5 vector from Addgene for Co-IP experiments. shRNA inserts were cloned into pLKO lentiviral vector (Addgene). ..

    Article Title: PHC1 maintains pluripotency by organizing genome-wide chromatin interactions of the Nanog locus
    Article Snippet: The coding sequence of Nanog gene was amplified from cDNA obtained from mESCs and cloned into the PiggyBac transposon pPB-CAG-3×FLAG-pgk-hph vector, a gift from Jin Zhang (Zhejiang University School of Medicine, China). .. HA-NANOG and FLAG-PHC1 sequences were cloned into pcDNA5 vector from Addgene for Co-IP experiments. shRNA inserts were cloned into pLKO lentiviral vector (Addgene). ..

    Article Title: mRNA-LNP vaccine-induced CD8 + T-cells protect mice from lethal SARS-CoV-2 infection in the absence of specific antibodies.
    Article Snippet: 547 HEK293T cells were placed in Opti-MEM® reduced serum media (Gibco) and 548 seeded in 12-well plates at 100k cells/well. .. Upon reaching ~70% confluency, cells were 549 transiently transfected with Spike Display B.1.1.7 cloned into pcDNA5 vector (Addgene 550 #172735) using 1.6 μg LipofectamineTM 3000 reagent (Invitrogen) and 1 μg DNA per well. ..

    Plasmid Preparation:

    Article Title: PHC1 maintains pluripotency by organizing genome-wide chromatin interactions of the Nanog locus.
    Article Snippet: The coding sequence of Nanog gene was amplified from cDNA obtained from mESCs and cloned into the PiggyBac transposon pPB-CAG-3×FLAG-pgkhph vector, a gift from Jin Zhang (Zhejiang University School of Medicine, China). .. HA-NANOG and FLAG-PHC1 sequences were cloned into pcDNA5 vector from Addgene for Co-IP experiments. shRNA inserts were cloned into pLKO lentiviral vector (Addgene). ..

    Article Title: PHC1 maintains pluripotency by organizing genome-wide chromatin interactions of the Nanog locus
    Article Snippet: The coding sequence of Nanog gene was amplified from cDNA obtained from mESCs and cloned into the PiggyBac transposon pPB-CAG-3×FLAG-pgk-hph vector, a gift from Jin Zhang (Zhejiang University School of Medicine, China). .. HA-NANOG and FLAG-PHC1 sequences were cloned into pcDNA5 vector from Addgene for Co-IP experiments. shRNA inserts were cloned into pLKO lentiviral vector (Addgene). ..

    Co-Immunoprecipitation Assay:

    Article Title: PHC1 maintains pluripotency by organizing genome-wide chromatin interactions of the Nanog locus.
    Article Snippet: The coding sequence of Nanog gene was amplified from cDNA obtained from mESCs and cloned into the PiggyBac transposon pPB-CAG-3×FLAG-pgkhph vector, a gift from Jin Zhang (Zhejiang University School of Medicine, China). .. HA-NANOG and FLAG-PHC1 sequences were cloned into pcDNA5 vector from Addgene for Co-IP experiments. shRNA inserts were cloned into pLKO lentiviral vector (Addgene). ..

    Article Title: PHC1 maintains pluripotency by organizing genome-wide chromatin interactions of the Nanog locus
    Article Snippet: The coding sequence of Nanog gene was amplified from cDNA obtained from mESCs and cloned into the PiggyBac transposon pPB-CAG-3×FLAG-pgk-hph vector, a gift from Jin Zhang (Zhejiang University School of Medicine, China). .. HA-NANOG and FLAG-PHC1 sequences were cloned into pcDNA5 vector from Addgene for Co-IP experiments. shRNA inserts were cloned into pLKO lentiviral vector (Addgene). ..

    shRNA:

    Article Title: PHC1 maintains pluripotency by organizing genome-wide chromatin interactions of the Nanog locus.
    Article Snippet: The coding sequence of Nanog gene was amplified from cDNA obtained from mESCs and cloned into the PiggyBac transposon pPB-CAG-3×FLAG-pgkhph vector, a gift from Jin Zhang (Zhejiang University School of Medicine, China). .. HA-NANOG and FLAG-PHC1 sequences were cloned into pcDNA5 vector from Addgene for Co-IP experiments. shRNA inserts were cloned into pLKO lentiviral vector (Addgene). ..

    Article Title: PHC1 maintains pluripotency by organizing genome-wide chromatin interactions of the Nanog locus
    Article Snippet: The coding sequence of Nanog gene was amplified from cDNA obtained from mESCs and cloned into the PiggyBac transposon pPB-CAG-3×FLAG-pgk-hph vector, a gift from Jin Zhang (Zhejiang University School of Medicine, China). .. HA-NANOG and FLAG-PHC1 sequences were cloned into pcDNA5 vector from Addgene for Co-IP experiments. shRNA inserts were cloned into pLKO lentiviral vector (Addgene). ..

    Polymerase Chain Reaction:

    Article Title: 2’,3’-Cyclic Nucleotide 3’-Phosphodiesterases Inhibit Hepatitis B Virus Replication
    Article Snippet: .. The PCR products were then digested with enzyme BamH I and Not I (Biolabs, Ipswich, Massachusetts) and inserted into the pCDNA5 vector (Addgene, Cambridge, MA) digested with the same enzymes. ..

    Transfection:

    Article Title: mRNA-LNP vaccine-induced CD8 + T-cells protect mice from lethal SARS-CoV-2 infection in the absence of specific antibodies.
    Article Snippet: 547 HEK293T cells were placed in Opti-MEM® reduced serum media (Gibco) and 548 seeded in 12-well plates at 100k cells/well. .. Upon reaching ~70% confluency, cells were 549 transiently transfected with Spike Display B.1.1.7 cloned into pcDNA5 vector (Addgene 550 #172735) using 1.6 μg LipofectamineTM 3000 reagent (Invitrogen) and 1 μg DNA per well. ..



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